Curriculum Vitae

 

Updated 4/04

Barry R. Lentz

 

 

I.BIOGRAPHICAL DATA

 

A.PERSONAL INFORMATION:

 

Business Address:                               418A Faculty Laboratory Office Building 231H

                                                           Department of Biochemistry and Biophysics

                                                           University of North Carolina School of Medicine

                                                           Chapel Hill, NC 27514

 

Business Telephone:                            Office: (919) 966-5384, Lab: 962-8317, FAX: 966-2852

 

E-Mail:                                                uncbrl@med.unc.edu

 

 

B.EDUCATION:

 

Postdoctoral:                                       National Institutes of Health Postdoctoral Fellow,

                                                           Department of Biochemistry,

                                                           University of Virginia, Charlottesville, VA 22901

                                                           Postdoctoral Mentor:  T.E. Thompson

                                                           July, 1973 - August, 1975.

 

Ph.D.:                                             Cornell University, Ithaca, NY; NIH Trainee

                                                           Physical and Biophysical Chemistry, August 1973.

                                                           Thesis:  A Structural Model for Liquid Water.

                                                           Advisor:  H. A. Scheraga, September, 1966 - June, 1973

 

B.A.:                                                   University of Pennsylvania, Philadelphia, PA;

                                                           Chemistry, May 1966

C.EMPLOYMENT HISTORY:

 

1996-1999                                         Faculty Director, UNC Macromolecular Interactions Facility

1995-present                                      Director, UNC Program in Molecular & Cellular Biophysics

1988-present                                      Professor of Biochemistry & Biophysics

                                                           418 Faculty Laboratory Office Building

                                                           University of North Carolina at Chapel Hill

                                                           School of Medicine

                                                           Chapel Hill, NC 27599-7260

1981-1988                                         Associate Professor

1975-1981                                         Assistant Professor

Jan.-July, 1972                                    Visiting Scientist with Professor H.A. Scheraga

                                                           Biophysics Department, Weizmann Institute of Science

                                                           Rehovot, Israel

1965 - 1966                                       Research Technician, Professor Phillip George

                                                           Department of Chemistry

                                                           University of Pennsylvania

                                                           Philadelphia, PA  19104

 

D.SPECIAL HONORS AND AWARDS:

 

Cum Laude with Honors, University of Pennsylvania

Phi Beta Kappa

Phi Lambda Upsilon

NSF Predoctoral Trainee, Cornell University

NIH Postdoctoral Fellow

American Heart Association Established Investigator; 1979-1984

University of North Carolina at Chapel Hill Excellence in Teaching Award, 2001 & 2002

Elected Fellow of American Association for the Advancement of Science, 2001

 

E.PROFESSIONAL SOCIETIES:

 

American Chemical Society, The Calorimetry Conference

American Society for Biochemistry and Molecular Biology, Biophysical Society

American Association for the Advancement of Science

II.TEACHING:

 

A.FORMAL TEACHING; PREVIOUS POSITION:

 

University of Virginia; Medical Biochemistry; 100 first year medical students; 3 recitations/wk; one semester, 1974; assisted Dr. T. E.Thompson.

 

B.FORMAL TEACHING; PRESENT POSITION

 

           1.         Biochemistry for Dental Hygiene Students (Biochemistry 7D); 50-60 students; 4-5 lectures/wk, 3 two-hour laboratory sessions and 5 two-hour recitations/semester; one semester/yr  1975-1977.

           2.         Nutrition for Dental Hygiene Students (Biochemistry 8D); 50-60 students; 2 lectures/wk; one semester/yr, 1975-1977.

           3.         Overview of Cell Biology for Dental Hygiene Students (Dental Hygiene 51); 50 students; 3 lectures/wk; one-third semester/yr, 1977-1980.

           4.         Membrane Biochemistry (Biochemistry 108); 15-25 advanced undergraduate or graduate students; 3 lectures/wk; one semester/yr, 1977-present; responsibility shared with Dr. G. Meissner; Dr. Lentz's contribution is currently 10 lectures on lipid chemistry and the dynamic structure of model and biological membranes.

           5.         Seminar in Membrane Structure (Biochemistry 230); 12 advanced graduate students; 2 hrs/wk; one semester/yr; 1975, 1977, 1979, 1981, 1987, 1989; responsibility shared with Dr. G. Meissner; participation by Drs. R.Penniall, P. Morell, and A. Erickson.

           6.         Physical Biochemistry (Biochemistry 133;144;143); 15 graduate students; 2-3 hrs/wk; one semester/yr; 1979-1994; responsibility shared with Dr. Jan Hermans; Dr. Lentz's contribution is 14 lectures in macromolecular structure and applications of spectroscopy to Biochemistry, alternating in different years between spin resonance spectroscopy and optical spectroscopy.

           7.         General Biochemistry (Biochemistry 100); 150 undergraduate students; 3 hrs/wk; one semester/yr; 1981, 1982, 8 lectures on lipid metabolism and oxidative phosphorylation.

           8.         Graduate Biochemistry Seminar (Biochemistry 205); 8-10 graduate students; Tutor and advise two students in the preparation of a seminar and coordinate the visit of a leading biochemist in the area of this seminar; one semester every other year 1979-present.

           9.         Experimental Approaches to Biochemical Research (Biochemistry 206); 8-12 graduate students; 2 hr lecture/demonstration per year; one semester per year, 1982-1989.

           10.       Biophysics (144, 145, 146, 147); planned and initiated a four module course as the core of the Biophysics Program; 1994-present.  Series director; 1994-present.  Teach two 15 hour modules: Basic Models in Structural Biology; Macromolecular Spectroscopy

11.              Biophysics 210/211: Biophysics seminar series and student seminar presentations; required of all students in the UNC Molecular and Cellular Biophysics Program.  Course preceptor and seminar series coordinator, 1995-present.

12.              Biophysics (150, 151, 152, 153, 154, 155, 156, 157, 158, 159); planned, initiated, and coordinate this eight module series that offer students introductions to such biophysical methods as x-ray crystallography, structural NMR, bioinformatics, molecular dynamics, analytical ultracentrifugation, cell imaging, mass spectroscopy, etc.  Series director 1998-present.

 

C.       GRADUATE AND POST-DOCTORAL RESEARCH TRAINING:

 

           1.         Current or past member of the Graduate Committee of twenty-nine students in the Biochemistry, Clinical Chemistry, Cell Biology, and Chemistry Ph.D. programs.

 

           2.         Candidates for Ph.D. degree currently in training: none

 

           3.         M.S. student trained:

                       Ms. Gail McIntyre, Biochemistry Department, received degree, 1989.

 

           4.         Ph.D.'s trained:

                       Bryant M. Moore, Vice President Shared Product Development, Medtronic Cardiac Rhythm Management, Minneapolis, MN, Ph.D. received 1980 from University of North Carolina at Chapel Hill.

                       Arthur P. Bode, Professor of Clinical Pathology and Diagnostic Medicine, East Carolina University School of Medicine, Greenville, NC.  Ph.D. received 1982 from University of North Carolina at Chapel Hill.

                       David A. Barrow, Lab Manager, Center for Oral and Systemic Disease, UNC-CH; Ph.D. received 1984 from Univ. of North Carolina at Chapel Hill.

                       Marcie Jones Hursting, Clinical Science Consulting, Potomac, MD.  Ph.D. received 1985 from University of North Carolina at Chapel Hill.

                       Roberta A. Parente, Senior Research Scientist, Ortho Diagnostic Systems (Johnson & Johnson), Raritan, NJ.  Ph.D. received 1985 from Univ. of North Carolina at CH.

                       Stephen W. Burgess, Vice President, R & D, Avanti Polar Lipids, Pelham, AL.  Ph.D. received 1990 from the University of North Carolina at Chapel Hill.

                       Susan Windes Tendian, was Research Scientist at Southern Research Institute in Birmingham, AL, currently unemployed to raise a family; Ph.D. received May 1991 from the University of North Carolina at Chapel Hill.

                       Gwyn A. Cutsforth, Research Scientist in a Triangle biotech firm; Ph.D. received May 1991 from the University of North Carolina at Chapel Hill.

                       Gang Pei, Professor and Head, Laboratory of Cellular Signal Transduction, Shanghai Institute of Cell Biology, Member Academia Sinica, Vice President of Chinese Society of Cell Biology;  Ph.D. received January, 1992 from the University of North Carolina at Chapel Hill.

                       Donald Massenburg, Professor of Internal Medicine, Lutheran Gen. Hosp., Parkridge, IL; Ph.D. received July, 1992 from the University of North Carolina at Chapel Hill.

            Qing Chen, Senior Staff Scientist at Amgen Corp., Camarillo, CA; Ph.D. received January, 1996, from Biochemistry & Biophysics Department, UNC-CH.

 

           5.         Research Associates trained:

                       Dr. T. E. Jensen (1979 – 1980) Ford Motor Company, Scientific Research Laboratory, Dearborn, MI  48121.

                       Dr. Daniel Powers,(March, 1986- September) 1989 currently a computer applications specialist at Nortel, Research Triangle Park, NC;.

                       Dr. Jogin Wu (1988 – 1993) Associate Director, Clinical Hematology Laboratory, Duke University Medical Center, Durham, NC.

                       Dr. Claudia Soodeen (1991-1992; 6 months) Research Scientist, Lederle-Praxis Biologicals.

                       Dr. Vishwanath Koppaka (1992-1996) after teaching math and science in a remote part of India for a year, he is currently a Research Assistant Professor, Dept of Pharmacology, Univ. of Penna., Philadelphia, PA.

                       Mr. Zheng Lian-Tsing (1993-1995) Visiting Scientist, National Laboratory of Biomolecules, Institute of Biophysics, Academia Sinica, Beijing, PR China.  Currently a graduate student in the Biochemistry & Biophysics Dept, UNC-CH.

                       Mr. Wu Hua (1993-1995) Visiting Scientist, Dept. of Biological Science & Biotechnology, Tsinghua Univ., Beijing, PR China.  Presently at CI Technology, Inc, Durham, NC

                       Dr. Wang Jian-Fang (1994–1997) Currently a Research Scientist at Dade Behring Diagnostics, Newark, DL.

                       Dr. Mou Banerjee, Research Associate, Dept of Biochemistry & Biophysics, Univ. of North Carolina at Chapel Hill, 1994-1997.  Currently seeking a position in India.

                       Dr. Jinkeun Lee (1995–1998) Senior Scientist, Transave Company, Princeton, NJ.

                       Dr. Zhai Xin (1996-1998) Independent consultant and networking engineer, San Jose, CA.

                       Dr. Kasturi Mukhopadhyay (1996–1997) Research associate in membrane biology; School of Life Sciences, Jawharlal Nehru University, New Delhi, India.

                       Dr. Szusza Lakos (1997–1999) Associate Professor, Dept. of Biophysics, Medical University of Pecs, Hungary.

                       Dr. Kervin Evans, (2003-present) Research Scientist, US Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, IL.

                       Dr. Arvind Srivastava (November, 1997 – December, 2001) Scientist, Medarex, Inc. W. Bloomsbury, NJ 08804

                       Dr. Vladimir Malinin (April, 1998 –January, 2001).  Scientist at Elan Pharmaceuticals, Inc., Princeton, NJ.

                       Dr. Emdadul Haque (June, 1998 – present) Research Associate, Dept of Biochemistry & Biophysics, Univ. of North Carolina at Chapel Hill.

                       Dr. Rinku Ghosh Majumder (April, 1998 – present) Research Associate, Dept of Biochemistry & Biophysics, Univ. of North Carolina at Chapel Hill.

                       Dr. Gabriel Weinreb (February, 1999 –June 2003) Research Assistant Professor, Dept of Cell & Developmental Biology, Univ. of North Carolina at Chapel Hill.

                       Dr. JinMing Huang (August, 1999–Sept. 2000) Research Associate, Dept of Chemistry, Wake Forest Univ., Winston Salem, NC.

                       Dr. Moses Dennison (April, 2001 – present) Research Associate, Dept of Biochemistry & Biophysics, Univ. of North Carolina at Chapel Hill.

 

D.       SPECIAL TEACHING:

 

           1.         Honors Research (Chemistry 99 or Honors 37); supervised undergraduate research of: Kenneth Morrison, 1977; Randall Davis, 1980-1981; Tamra Carpenter, 1981-1982; Sherman Madden, 1982; Robert Whitaker, 1985-1986; Mohak Davé, Spring,1994.

           2.         Supervise or have supervised laboratory research of several other undergraduate students with chemistry, biology, or physics majors, 1976-present.

           3.         Advisor to a high school student in UNC Summer Research Apprentice Program for minority students, 1980.

4.                  Biochemistry 207. Advanced Biochemistry Laboratory.  Eight graduate students; one-half semester per student; 1975-1997.

5.                  Pharmaceutics 163. Advances in Drug Delivery. One lecture per year on the mechanism of membrane fusion; 1992-present.

6.                  Supervised research and arranged and sponsored a research visit to Beijing Medical University for Dr. Jeffrey Simko, Ph.D. in analytical chemistry and a medical student at UNC-CH; fall, 1996, spring & summer, 1997.

 

III.      PROFESSIONAL AND COMMUNITY SERVICE ACTIVITIES:

 

A.       EXTRA-UNIVERSITY SERVICE:

 

           1.         Peer Review

 

                       National Science Foundation Peer Reviewer, (Biophysics, International Program,          Biochemistry, Physiology).

                       Peer Reviewer for United States-Israel Binational Science Foundation

                       Peer Reviewer for March of Dimes Birth Defects Foundation

                       Ad Hoc Member of Molecular and Cellular Biophysics Study Section of National          Institutes of Health, 1984

                       Member of a Special Study Section of the National Institute of Health to review a          challenge to a Molecular and Cellular Biophysics Study Section Review,         April,    1985.

                       National Science Foundation EPSCoR ("Experimental Program to Stimulate      Competitive Research") Panel Member, May, 1986

                       External reviewer for the Physical Biochemistry Study Section of National Institutes       of Health, 1987.

                       National Science Foundation Biophysics Program Panel Member, July, 1987 - June,     1988

                       Member of the National Institutes of Health Biophysics and Biochemistry-B Study         Section, July, 1988 - 1992.

                       Site-visit review of "Cell Imaging Laboratory" Special Resource for the NIH, July,         1991.

                       National Institutes of Health Reviewer Reserve, July, 1992- June 1996.

                       NIH Promotion Committee for Dr. Leonid Chernomordik, 1997.

                       National Institutes of Health Special Emphasis Panel (Biomedical Research Training), Nov, 1997, site visit to Univ. of Mich., Oct, 1997.

                       NIH, Special Study Section for NRSA awards, March 23-24, 1998.

 

           2.         Professional Journal Review

 

                       Reviewer for Biochemistry, Analytical Biochemistry, Biophysical Journal, Nature, Archives of Biochemistry and Biophysics, Journal of Biological Chemistry, Journal of Biochemical and Biophysical Methods, and Blood

 

           Editorial Board Member, Journal of Fluorescence, 1992-1998.  Managing editor of a special volume on "Fluorescent Methods for Resolving Microenvironments in Membranes, 1996."

 

                       Editorial Board Member, Biophysical Journal, July, 1998-2002.

 

                       Associate Editor, Biophysical Journal, June 2002-present.

 

           3.         Organization of Scientific Meetings

 

                       Organizer of "Liposomes in Space" Workshop at the 1986 Biophysical Society             Meeting.

                       Fund-Raising Chairman and Co-Chairman of the Organizing Committee for       "Fundamental Mechanisms of Membrane Fusion" Symposium held at the

                                   International Biophysics Congress, Jerusalem, ISRAEL, 1987.

                       Chair of Session "Formation and Function of Platelet Micro-particles" at the 9th             National Conference on Thrombosis and Hemostasis, American Heart           Association Meetings, Dallas, 1990.

                       Organizer of a Symposium on "Protein-Lipid Interactions and the Stabilization of            Membrane Proteins" at the 1993 Calorimetry Conference held at Duke         University, Durham, NC, 7/18/93-7/23/93.

                       Co-Organizer of "Mechanisms of Biological Membrane Fusion" satellite symposium held in conjunction with the International Congress of Pure and Applied Biophysics, August 8-10, 1996, Noorwijkerhout, The Netherlands.

                       Chair of the Organizing Committee for "Membrane Molecular Dynamics", the first International Triangle Biophysics Symposium jointly sponsored by UNC Molecular & Cellular Biophysics and Duke Molecular Biophysics Graduate Programs; October 24-26, 1996, Chapel Hill, NC.

                       Organizer of a joint workshop (Membrane Structure & Assembly and Biological Fluorescence Subgroups) on “Applications of Fluorescence Imaging in Cell Membrane Biophysics” at the 1998 Biophysical Society meeting, Kansas City.

                        Member of the Organizing Committee for the second International Triangle Biophysics Symposium jointly sponsored by UNC Molecular & Cellular Biophysics and Duke Molecular Biophysics Graduate Programs; Nov 19-21, 1998 at the Durham Civic Center) on “Principles of Protein Design: Theory and Applications.”

Session (“Fusion Techniques and Mechanisms”) Chair for “Membrane Fusion: Mechanisms and Applications to Cell Biology, Drug Delivery, and Gene Therapy.” Symposium, Salamanca, Spain, July 14-18, 1998.

Organizer of “Phospholipases and Sphingomyelinases: Enzymology at a Surface”, Membrane Structure and Assembly Subgroup Symposium at the Biophysical Society meeting, New Orleans, LA, February 12, 2000.

Chair of Organizing Committee for “Macromolecular Interactions: Emerging Concepts and Technologies”, the third International Triangle Biophysics Symposium jointly sponsored by UNC Molecular & Cellular Biophysics and Duke Molecular Biophysics Graduate Programs; October 19-20, 2000, Chapel Hill, NC.

Symposium organizer 2003 Biophysical Society national meeting: Molecular Mechanisms of Membrane Fusion: Protein Machines & Lipid Materials

 

           4.         Participation in Professional Societies

 

Member of the Congressional Liaison Committee of the Biophysical Society, 1992-present.

Member of the Advisory Committee of the Membrane Structure and Assembly Subgroup of the Biophysical Society, 1995-2000.

Chair of the Membrane Structure and Assembly Subgroup of the Biophysical Society, 1999.

Subgroup Liaison for the Biophysical Society Administration, 2000.

Elected Member of the Biophysical Society Council, October, 2001-present.

Chair of Biophysical Society Minority Affairs Committee, March 2002-present.

 

           5.         Consulting

          

                       Consultant for American Dade, Miami, FL., 1983-1987; prethrombotic state.

 

                       Consultant for Miles Laboratories, Elkhart, IN, 1986, 1989; separations of amphipathic compounds.

 

                       Consultant for Burroughs-Welcome Research Laboratories, Research Triangle Park, NC, 1988-1995; fluorescence & DSC studies of reverse transcriptase domain structure.

 

                       Consultant for Ortho Diagnostic Systems, Raritan, NJ, 1991; liposome technology.

 

                       Consultant for Organon Teknika Corporation, Durham, NC, 1988-1991; surface phenomena in SPIA assays.

 

           6.         Current Community Service

 

                       President of the Sunset Beach Taxpayers' Association representing 1500 property owners in Sunset Beach, NC in dealings with municipal, state, and federal government representatives and agencies.

 

                       Stephen minister and ministerial supervisor at the University United Methodist Church, Chapel Hill, NC.  Stephen ministers are trained Christian caregivers and supplement the services offered by professional ministerial staff.

 

                       President of the Chapel Hill-Carrboro Adult Soccer Association that has for 30 years organized coeducational, mixed-age and multi-ethnic-group recreational soccer matches for the Chapel Hill-Carrboro and surrounding communities.

 

 

B.       UNIVERSITY SERVICE:

 

           1.         Committees

 

                       Member of the Dental Hygiene Academic Performance Committee, 1977-1986.

 

                       Member of Search Committee for Director of Dental Hygiene, 1979-1980.

 

                       Program Review Committee of the Graduate School, 1992-present.

 

                       SACS: Member of the Task Force on Graduate & Professional Programs, 1993-1994.

                       Chairman of a subcommittee preparing a report on interdisciplinary studies.

 

                       Member of the Acting Dean's Advisory Committee on the Reorganization of the

                       Graduate School, Feb, 1995-1996.

 

                       Member of three Search Committees to recruit an Associate and two Assistant Deans of the Graduate School; Spring, 1995.

 

                       Member, Agenda Committee of the Faculty Council, 1997.

 

                       Member, Graduate School Committee on Conflict of Interest, 1997.

 

                       Member, Advisory Committee to the Curriculum in Applied Sciences, 1997-2000.

 

                       Member, Director Search Committee, Curriculum in Applied Science, Spring, 1998.

 

                       Member, Biophysics faculty search Committee, Physics Department, January, 2002-present.

 

                       Member of the Program Review Committee for the Physics Department, March, 2002.

 

           2.         Contributions to the Scholarly Community

 

                       Organizer and coordinator of UNC-Duke Biomembrane Structure Club with thirteen faculty participants from the Biochemistry, Anatomy, and Physiology Departments at both institutions; 1976 - 1996.

 

Organizer of Coagulation Journal Club with student and faculty participants from Biochemistry, Chemistry, Pathology, and Medicine Departments.  Focuses on role of platelets in coagulation in general and specifically on prothrombin and Factor X activation; 1982-1989.

 

                       Organized the visit of Dr. F.A. Dombrose of Organon Teknika Corporation as the N.C. Biotechnology Center Visiting Industrial Scientist at UNC, Spring, 1987.

 

                       Arranged & coordinated the Wellcome Visiting Professorship of Professor Lin Ke-Chun, Director of the Biophysics Department of Beijing Medical University, P.R. of China; 2/26/93-3/6/93.

 

Originator & coordinator of the Beijing/Chapel Hill Biophysics Exchange Program, funded by The UNESCO Executive Committee for Molecular and Cell Biology; 1993- present.

 

                       Organizer of the Surfaces in Hematology Club with trainee and faculty participants from five departments at UNC Chapel Hill; 1994-1996.

                      

           3.         Administration

 

                       Elected representative to the UNC faculty council; 1977-1980; 1994-2000.

 

                       Proposed, organized, chaired the planning committees for, and implemented a Graduate Training Program in Molecular & Cellular Biophysics at UNC, 12/91- 4/95.  Director of the Program 4/95-present.

 

                       Member of the Administrative Board of the Graduate School, 1992-1998.  Chair of a subcommittee to review student appeals, 1993.

 

                       Founder and Faculty Director of the Macromolecular Interactions Facility that provides to UNC and Research Triangle area laboratories inter-related biophysical methods used to characterize the interactions between and solution structure of biological macromolecules.  1996-1999.

 

C.       SCHOOL OF MEDICINE SERVICE:

 

           Sub-committee of Faculty Promotions Committee, 1982.

 

           Faculty Panel to interview candidates for Associate Dean for Administration; 1985.

 

           Ad hoc steering committee appointed by the Dean of the School of Medicine to initiate and facilitate discussions between UNC and Organon-Teknika Corporation on mutual interests in the area of biomedical research and development, Fall, 1987.

 

           Thrombosis and Hemostasis Advisory Committee, July 1988.

 

           Member, Search Committee for the Physiology Chair, December 1989 to 1991.

 

           Subcommittee on Graduate Education in Basic Sciences for reaccreditation by the Liaison                       Committee on Medical Education (LCME), 1996.

 

           Chair of the Faculty Council Nomination Committee for the School of Medicine, 1997.

 

           Member and Co-Chair of Basic Sciences Review Group, Post-tenure Review Committee, School of Medicine, 1999-2000.

 

D.       DEPARTMENT OF BIOCHEMISTRY SERVICE:

 

           1.         Committees

 

                       Member of the Biochemistry Chairman's committee to reorganize the graduate curriculum in Biochemistry, 1977.

 

                       Chairman of a committee to establish curriculum and requirements for the Master of Science in Biochemistry, 1977.

 

Member of the Biochemistry Chairman's committee to evaluate the physical chemistry requirements of the Biochemistry Ph.D. degree program; 1978-1983.

 

                       Member of the Biochemistry Department Library Committee, 1978 - present.

 

                       Member of the Biochemistry Seminar Program Committee; 1977 - 1982.

 

                       Member of the Biochemistry Graduate Recruitment and Admissions Committee;

                       1979 - 1991.

 

                       Member of the Biochemistry Equipment Committee; 1978-1982.

 

                       Member of the Biochemistry Graduate Admissions & Advisory Committee; 1985-1995.

 

                       Member of the Biochemistry Department Space Committee; 1986.

 

                       Chair of the Biochemistry Department Ad Hoc Committee on Student Grant Proposal Guidelines; 1987.

 

Member of the Biochemistry Department Committee on Joint Appointment Policy; 1987, 1989.

 

Member of the Biochemistry Department Committee on Graduate Curriculum Change; 1988.

 

Member of the Biochemistry Department Ad Hoc Committee on Revision of Student Grant Proposal Guidelines, 1988.

 

Member of Biochemistry Recruitment Committee for a position in Structural Basis of Cooperative Enzymatic Mechanisms, Nov, 1990 to June, 1991.

 

                       Chair of the Graduate Curriculum Review Committee, 11/91-11/92.

 

                       Member of Biochemistry Recruitment Committee for a position in Structure Determination by NMR, 1992.

 

                       Member, Graduate Program Advisory Committee, 1996-1998.

 

Member of the Biochemistry Department Ad Hoc Committee on Revision of Student Grant Proposal Guidelines, 1998; 2000.

 

Chair of Faculty-recruiting committee to recruit two biophysicists to the Department of Biochemistry & Biophysics, September 2001 – April 2002.

 

           2.  Administration

 

                       Organizer and faculty advisor for the Biochemistry Student-sponsored Invited Lecturer Program; 1977-1982.

 

                       Organizer of weekly luncheons for students and invited speakers; 1977-1982.

 

                       Associate Director of Graduate Studies, Department of Biochemistry and Nutrition; 1985-1991.

III.      RESEARCH

 

A.       MAJOR AREAS OF RESEARCH INTEREST:

 

           In general, my research has applied the techniques and concepts of physical chemistry to the solution of biologically relevant problems.  My thesis research involved a theoretical treatment of the structure of water and aqueous solutions with the purpose of gaining insight into the effect of water on protein structure.  Since completing my formal training, I have been engaged in experimental studies that aim to determine the molecular basis for the physical and biological properties of increasingly complex membrane systems.  In these studies, I have used fluorescence spectroscopy, nuclear magnetic resonance spectroscopy, Fourier transform infrared and CD spectroscopy, microcalorimetry, electron microscopy, enzyme kinetics, molecular biological protein manipulations, standard biochemical techniques, and the concepts of statistical thermodynamics to define the relationship of membrane and protein structure to biological function.  My overriding goal has been to apply the basic principle of molecular structure and dynamics to the solution of practical problems in the biomedical sciences.

           In one project, my laboratory seeks to define the mechanisms by which platelet-membrane phosphatidylserine (PS) regulates thrombin formation during blood coagulation.  This project started with studies of membrane morphological and biochemical changes associated with human platelet stimulation.  We were the first to show that PS, which appears mainly on the cytoplasmic surface of resting platelet membranes, appears on the plasma-exposed surface of membrane vesicles that derive from human platelets upon their activation and that these vesicles were needed for prothrombin activation to thrombin during blood coagulation.  Currently, our work focuses on biophysical studies of the membrane assembly and lipid-regulation of the enzyme-cofactor complex that activates prothrombin to thrombin.  We were the first to report a specific requirement for PS as opposed to other negatively charged lipids, and we hypothesized that PS might be a specific regulator of blood coagulation.  We have now shown that PS allosterically up-regulates the enzyme that activates prothrombin, enhances the ability of the cofactor for this reaction to bind the enzyme and alters the conformation of the substrate.  Thus, PS is the second messenger that turns on thrombin generation.  We have located two regulatory sites on prothrombin, two on the enzyme, and four on the cofactor.  We have located the two sites on prothrombin, narrowed the location of the two sites on the enzyme, and located one of the four sites on the cofactor.  One current effort is to define the lipid specificity of these sites and ultimately to define the structure of these PS-sensitive regulatory sites.  We are also seeking to understand how one or more of the regulatory sites on the cofactor might be linked to a wide-spread mutation that is clearly correlated with thrombotic disease.

           Another project aims to establish in model membranes the molecular mechanism by which poly(ethylene glycol) (PEG) induces membrane fusion.  We started this work as a means to understand how PEG facilitated fusion of cell membranes to create hybrid cells, but have more recently found that PEG-mediated of pure lipid synthetic membranes is an excellent model for much more complex, protein-mediated cellular fusion events.  We have proposed an hypothesis that describes the sequence of molecular reorganizations needed to accomplish fusion of juxtaposed lipid bilayers.  This hypothesis accounts for the sequence of electrical changes observed during fusion between cell compartments as well as for the large activation energies associated with the cellular fusion process.  As such, it provides strong evidence that cell membrane fusion is lipidic in its basic mechanism.  We are currently examining the details of this mechanism both to test it experimentally and to explore how proteins might catalyze the rate-limiting individual steps of the process.  We plan to extend the methods we developed for these studies to examine the mechanism by which the simplest and best understood cellular fusion machine, the influenza virus protein hemagglutinin, induces fusion of viral membrane to the endosome membrane during viral infection.  We will also examine the effect of the basic machinery of exocytotic fusion (the “core complex”) on fusion of our model membranes.  Ultimately, we hope to use this information to find methods for better delivering drug-laden liposomes to and inducing their fusion with target cells.

 

 

B.       GRANTS AWARDED (or pending for likely funding):

 

           1.         Principal Investigator

                       a.         National Institutes of Health GM32707-17 to 21

                       b.         "Microstructural Heterogeneity in Membranes"

                       c.         $210,000 first year

                       d.         4/1/02 to 3/31/06

e.                   30% effort

 

           2.         Principle Investigator

                       a.         National Institutes of Health HL072827

                        b.         “Lipid Regulation of Thrombin Generation”

                        c.         $328,526 requested; $275,000 recommended

                        d.         7/1/04 to 6/30/08; pending approval by Council (20%tile score)

                        e.         30% effort

 

           3.         Principle Investigator

                       a.         National Institutes of Health 1T32GM08570-01

                       b.         "Molecular and Cellular Biophysics Training Program"

                       c.         6 positions per year; renewal request for 12 per year

                       d.         7/1/95 to 6/30/05.

                       e.         20% effort

 

C.       PUBLICATIONS:

 

           In Refereed Journals:

           1.         George P, Witonsky RJ, Trachtman M, Wu C, Dorwart W, Richman L, Richman          W, Shuravh F, Lentz B:  An Enquiry into the Importance of Solution Effects in                        Phosphate Ester and Anhydride Reactions.  Biochim Biophys Acta 223:1-15 1970.

 

           2.         Lentz BR, Scheraga HA:  Water Molecule Interactions: Stability of Cyclic Water           Polymers.  J Chem Phys 58:5296-5308, 1973.

 

           3.         Lentz BR, Hagler AT, Scheraga HA:  Structure of Liquid Water. II. Improved Statistical Thermodynamic Treatment and Implications of a Cluster Model.  J Phys Chem 78:1531-1550, 1974.

 

           4.         Lentz BR, Hagler AT, Scheraga HA:  Vibrational Frequencies of Water Clusters.  J Phys Chem 78:1844-1847, 1974.

 

           5.         Lentz BR, Barenholz Y, Thompson TE:  A Simple Method for the Synthesis of Cholesterol Esters in High Yield.  Chem Phys Lipids 15:216-221, 1975.

 

           6.         Owicki JC, Lentz BR, Hagler AT, Scheraga HA:  Structure of Liquid Water.III.  Thermodynamic Properties of Liquid Deuterium Oxide. J Phys Chem 79:2352-2361, 1975.

 

           7.         Suurkuusk J, Lentz BR, Barenholz Y, Biltonen RL, Thompson TE:  A Calorimetric and Fluorescent Probe Study of the Gel-Liquid Crystalline Phase Transition in Small, Single-Lamellar Dipalmitoyl Phosphatidylcholine Vesicles.  Biochemistry 15:1393-1401, 1976.

 

           8.         Lentz BR, Barenholz Y, Thompson TE:  Fluorescence Depolarization Studies of Phase Transitions and Fluidity in Phospholipid Bilayers, I.  Single Component Phosphatidylcholine Liposomes.  Biochemistry 15:4521-4528,1976.

 

           9.         Lentz BR, Barenholz Y, Thompson TE:  Fluorescence Depolarization Studies of Phase Transitions and Fluidity in Phospholipid Bilayers. II. Two-Component Phosphatidylcholine Liposomes.  Biochemistry 15:4529-4537, 1976.

 

           10.       Roseman M, Lentz BR, Sears B, Gibbs D, Thompson TE:  Properties of Sonicated Vesicles of Three Synthetic Phospholipids.  Chem Phys Lipids 21:205-222,1978.

 

           11.       Shaw JM, Hutton WC, Lentz BR, Thompson TE:  Proton NMR Study of the Decay of Transbilayer Compositional Asymmetry Generated by a Phosphatidylcholine Exchange Protein.  Biochemistry 16:4156-4163, 1977.

 

           12.       Lentz BR, Freire E, Biltonen RL:  Fluorescence and Calorimetric Studies of Phase Transitions in Phosphatidylcholine Multilayers: Kinetics of the Pretransition.  Biochemistry 17:4475-4480, 1978.

 

           13.       Lentz BR, Litman BJ:  Effect of Head Group on Phospholipid Mixing in Small, Unilamellar Vesicles: Mixtures of Dimyristoyl Phosphatidylcholine and Dimyristoyl Phosphatidylethanolamine.  Biochemistry 17:5537-5543,1978.

 

           14.       Moore BM, Lentz BR, Meissner G:  Effects of Sarcoplasmic Reticulum Ca2+-ATPase on Phospholipid Bilayer Fluidity: Boundary Lipid. Biochemistry 17:5248-5255, 1978.

 

           15.       Lentz BR, Moore BM, Barrow DA:  Light-Scattering Effects in the Measurement of Membrane Microviscosity with Diphenylhexatriene. Biophysical J 25:489-494, 1979.

 

           16.       Lentz BR, Alford DR, Dombrose FA:  Determination of Phosphatidylglycerol Asymmetry in Small, Unilamellar Vesicles by Covalent Labelling. Biochemistry 19:2555-2559, 1980.

 

           17.       Barrow DA, Lentz BR:  Large Vesicle Contamination in Small, Unilamellar Vesicles.  Biochim Biophys Acta 597:92-99, 1980.

 

           18.       Lentz BR, Barrow DA, Hoechli M:  Cholesterol-Phosphatidylcholine Interactions in Multilamellar Vesicles.  Biochemistry 19:1943-1954, 1980.

 

           19.       Bode AP, Dombrose FA, Lentz BR, Roberts HR:  The Platelet Membrane as a Catalytic Surface in Thrombin Generation: Availability of Platelet Factor  1 and Platelet Factor 3.  N Y Acad Sci 370:348-358, 1981.

 

           20.       Barrow DA, Lentz BR:  A Model for the Effect of Lipid Oxidation on Diphenylhexatriene Fluorescence in Phospholipid Vesicles. Biochim Biophys Acta 645:17-23, 1981.

 

           21.       Dombrose FA, Bode AP, Lentz BR:  Differentiation of Factor V-like Coagulant Activity from Catalytic Phospholipid-like Surface Activity in Membrane Fractions Derived from Human Platelets.  Thrombosis Research 22:603-621,1981.

 

           22.       Lentz BR, Moore BM, Kirkman C, Meissner G:  Lipid-Protein Interactions in Sarcoplasmic Reticulum: A Disrupted Secondary Lipid Layer Surrounds the Ca2+-ATPase.  Biophysical J 37:30-32, 1982.

 

           23.       Moore BM, Lentz BR, Hoechli M, Meissner G:  Effect of Lipid Membrane Structure on the ATP Hydrolyzing Activity of the Calcium-Stimulated Adenosine-Triphos­phatase of Sarcoplasmic Reticulum. Biochemistry 20:6810-6817, 1981.

 

           24.       Lentz BR, Hoechli M, Barenholz Y:  Acyl Chain Order and Lateral Domain Forma­tion in Mixed Phosphatidylcholine-Sphingomyelin Multilamellar and Unila­mel­lar Vesicles.  Biochemistry 20:6803-6809, 1981.

 

           25.       Lentz BR, Alford DR, Hoechli M, Dombrose FA:  Phase Behavior of Mixed Phospha­tidylglycerol-Phosphatidylcholine Multilamellar and Unilamellar Vesicles.  Biochemis­try 21:4212-4219, 1982.

 

           26.       Lentz BR, Madden S, Alford DR:  Trans-Bilayer Redistribution of Phosphatidyl­glycerol in Small, Unilamellar Vesicles Induced by Specific Divalent Cations.  Biochemistry 21:6799-6807, 1982.

 

           27.       Barrow DA, Lentz BR:  The Use of Isochronal Reference Standards in Phase and Modulation Fluorescence Lifetime Measurements.  J Biochem Biophys Meth 7:217-234, 1983.

 

           28.       Lentz BR, Clubb KW, Barrow DA, Meissner G:  Ordered and Disordered Phos­pholipid Domains Coexist in Membranes Containing the Calcium Pump Protein of Sarcoplasmic Reticulum.  Proc Natl Acad Sci USA 80:2917-2921, 1983.

 

           29.       Barrow DA, Lentz BR:  Quinine as a Fluorescence Lifetime Standard: Conditions for Effectively Homogeneous Decay.  Chem Phys Letts 104:163-167, 1984.

 

           30.       Parente RA, Lentz BR:  Phase Behavior of Large Unilamellar Vesicles Composed of Synthetic Phospholipids. Biochemistry 23:2353-2362, 1984.

 

           31.       Lentz BR, Clubb KW, Alford DR, Hoechli M, Meissner G:  Phase Behavior of Membranes Reconstituted from Dipentadecanoyl Phosphatidylcholine and the Mg2+-de­pendent, Ca2+-stimulated Adenosine triphosphatase of Sarcoplasmic Reticulum:  Evi­dence for a Disrupted Lipid Domain Surrounding Protein. Bio­chemistry 24:433-442, 1985.

 

           32.       Parente RA, Hoechli M, Lentz BR:  Morphology and Phase Behavior of Two Types of Unilamellar Vesicles Prepared from Synthetic Phosphatidylcholines:  A Freeze-Fracture and Calorimetric Study. Biochem. Biophys. Acta. 812:495-502,1985.

 

           33.       Sandberg H, Bode AP, Dombrose FA, Hoechli M, Lentz BR:  Expression of Coagu­lant Activity in Human Platelets: Release of Membranous Vesicles Providing Platelet Factor 1 and Platelet Factor 3.  Thrombos. Res. 39:63-79, 1985.

 

           34.       Bode AP, Sandberg H, Dombrose FA, Lentz BR:  Association of Factor V Activity with Membranous Vesicles Released from Human Platelets: Requirement for Platelet Stimula­tion.  Thrombosis Res. 39:49-61, 1985.

 

           35.       Barrow DA, Lentz BR:  Membrane Structural Domains:  Resolution of Heteroge­neous Diphenylhexatriene Fluorescence Using Phase and Modulation Decay Measurements.  Biophys. J. 48:221-234, 1985.

 

           36.       Parente RA, Lentz BR:  Advantages and Limitations of 1-palmitoyl-2-(4'-carboxy­ethyl)-6-diphenyl-trans-1,3,5-hexatriene)-3-sn-phosphatidylcholine as a Fluorescent Membrane Probe.  Biochemistry 24:6178-6185, 1985.

 

           37.       Lentz BR, Alford DR, Jones ME, Dombrose FA:  Calcium-Dependent and Indepen­dent Interactions of Prothrombin Fragment 1 with Phosphatidylglycerol/­Phosphatidyl­choline Unilamellar Vesicles. Biochemistry 24:6997-7005,1985.

 

           38.       Jones ME, Lentz BR, Dombrose FA, Sandberg H:  Comparison of the Abilities of Synthetic and Platelet-Derived Membranes to Enhance Thrombin Formation.  Throm­bosis Res. 39:711-724, 1985.

 

           39.       Jones ME, Griffith MJ, Monroe DM, Roberts HR, Lentz BR:  Comparison of Lipid -Binding and Kinetic Properties of Normal, Variant, and Gla - Modified Human Factor IX and Factor IXa.  Biochemistry 24:8064-8069, 1985.

 

           40.       Jones ME, Lentz BR:  Phospholipid Lateral Organization in Synthetic Membranes as Monitored by Pyrene-Labeled Phospholipids:  Effects of Temperature and Prothrombin Fragment 1 Binding.  Biochemistry 25:567-574, 1986.

 

           41.       Parente RA, Lentz BR:  Fusion and Phase Separation Monitored by Lifetime Changes of a Fluorescent Phospholipid Probe.  Biochemistry 25:1021-1026, 1986.

 

           42.       Parente RA, Lentz, BR:  Rate and Extent of Poly(ethylene glycol)-Induced Large Vesicle Fusion Monitored by Bilayer and Internal Contents Mixing. Biochemistry 25:6678-6688, 1986.

 

           43.       Lentz BR, Carpenter TJ, Alford DR:  Spontaneous Fusion of Phosphatidylcholine Small Unilamellar Vesicles in the Fluid Phase. Biochemistry 26: 5389-5397, 1987.

 

           44.       Lentz BR, Burgess SW:  A Dimerization Model for the Concentration Dependent Photophysical Properties of Diphenylhexatriene and Its Phospholipid Derivatives: DPHpPC and DPHpPA.  Biophysical J. 56:723-733, 1989.

 

           45.       Lentz BR, Whitt NA, Alford DR, Burgess SW, Yates JC, Nir S:  The Kinetic Mecha­nism of Cation Catalyzed Phosphatidylglycerol Transbilayer Migration Implies Close Contact Between Vesicles as an Intermediate State. Biochemistry, 28:4575-4580, 1989.

 

           46.       Cutsforth GA, Whitaker RN, Hermans J, Lentz BR:  A New Model to Describe Extrinsic Protein Binding to Phospholipid Membranes of Varying Composition: Applica­tion to Human Prothrombin and Factor X. Biochemistry 28:7453-7459, 1989.

 

           47.       Lentz BR, Hermans J:  A New Model to Describe Extrinsic Protein Binding to Phospho­lipid Membranes of Varying Composition: Quantitative Treatment.  Biochem­istry 28:7459-7461, 1989.

 

           48.       Lentz BR:  Membrane "Fluidity" as Detected by Diphenylhexatriene Probes Chem. Phys. Lipids 50:171-190, 1989.

 

           49.       Tendian SW, Lentz BR:  Evaluation of Membrane Phase Behavior as a Tool to Detect Extrinsic Protein-Induced Domain Formation:  Binding of Prothrombin to Phosphati­dylserine/Phosphatidylcholine Vesicles.  Biochemistry 29:6720-6729, 1990.

          

           50.       Pei G, Baker K, Emfinger SM, Fowlkes DM, Lentz BR:  Expression, Isolation, and Characterization of an Active Site (SER528 ® ALA) Mutant of Recombinant Pro­thrombin. J. Biol. Chem. 266, 9598-9604, 1991.

 

           51.       Pei G, Lentz BR:  Isolation and Characterization of Human Meizothrombin in the Presence of Danzylarginine N-(3-Ethyl-1,5-Pentanediyl)Amide. Blood Coagulation & Fibrinolysis 2, 309-316, 1991.

 

           52.       Wu JR, Lentz, BR: Mechanism of Poly(ethylene glycol)-Induced Lipid Transfer Between Phosphatidylcholine Large Unilamellar Vesicles: A Fluorescent Probe Study. Biochemis­try, 30, 6780-6787, 1991.

 

           53.       Burgess SW, Massenburg D, Yates J, Lentz BR:  Poly(ethylene glycol)-Induced Lipid Mixing but Not Fusion between Synthetic Phosphatidylcholine Large, Unilamellar Vesicles. Biochemistry 30, 4193-4200, 1991.

 

           54.       Wu JR & Lentz BR: Fourier Transform Infrared Spectroscopic Study of Ca2+- and

                       Membrane-Induced Secondary St­ruct­ural Changes in Bovine Prot­hrombin and Prot      hrombin Fragment 1. Bi­ophys. J., 60, 70-80, 1991.

 

           55.       Lentz BR, Wu JR, Sorrentino AM, Carleton JN: Membrane Binding Induces Lipid-Specific Changes in the Denaturation Profile of Bovine Prothrombin: A Scanning Calorimetric Study. Biophys. J., 60, 942-951, 1991.

 

           56.       Tendian SW, Lentz BR, Thompson NL: Evidence from Total Internal Reflection Fluorescence Microscopy for Calcium-Independent Binding of Prothrombin to Nega­tively Charged Planar Phospholipid Membranes. Biochemistry, 30, 10992-10999, 1991.

 

           57.       Burgess SW, Wu JR, Swift K, Lentz BR: Determination of the Rate of Rapid Lipid Transfer Induced by Poly(ethylene glycol) Using the SLM Fourier Transform Phase and Modulation Spectrofluorometer. J. of Fluorescence, 1, 105-112, 1991.

 

           58.       Lentz BR, McIntyre GF, Parks, DJ, Yates JC, Massenburg D: Bilayer Curvature and Certain Amphipaths Promote Poly(Ethylene Glycol) Induced Fusion of Dipalmitoylph­osphatidylcholine Unilamellar Vesicles. Biochemistry 31, 2643-2653, 1992.

 

           59.       Burgess SW, McIntosh TJ, Lentz BR: Modulation of Poly(Ethylene Glycol)-Induced Fusion by Membrane Hydration: Importance of Interbilayer Separation. Biochemistry, 31, 2653-2661, 1992.

 

           60.       Pei G, Laue TM, Aulabaugh A, Fowlkes DM, Lentz BR: Structural Comparisons of Meizothrombin and Its Precursor Prothrombin in the Presence or Absence of Procoag­ulant Membranes. Biochemistry, 31, 6990-6996, 1992.

 

           61.       Pei G, Powers DD, Lentz BR: Specific Contribution of Different Phospholipid   Surfaces to the Activation of Prothrombin by the Fully Assembled Prothrombinase. J. Biol. Chem. 268: 3226-3233, 1993.

 

           62.       Powers, DD, Lentz, BR: Simulation of Prothrombin Proteolysis by the Full Prothrom­binase Assembled on Varied Phospholipid Surfaces. J. Biol. Chem. 268, 3234-3237, 1993.

 

           63.       Massenburg, D, Lentz, BR: Poly(Ethylene glycol)-Induced Fusion and Rupture of Dipalmitoylphosphatidylcholine Large, Unilamellar Extruded Vesicles. Biochemistry, 32, 9172-9180, 1993.

 

           64.       Pearce KH, Hof M, Lentz BR, Thompson NL: Comparison of the Membrane Binding Kinetics of Bovine Prothrombin and Its Frag­ment1.  J. Biol. Chem. 268, 22984-22991, 1993.

 

           65.       Lentz, BR: Use of Fluorescent Probes to Monitor Molecular Order and Motions within Liposome Bilayers.  Chem. Phys. of Lipids 64: 99-116, 1993.

 

           66.       Wu JR & Lentz BR: Phospholipid-Specific Conformational Changes in Human Pro­thrombin upon Binding to Procoagulant Acidic Lipid Membranes. Thrombosis & Haemostasis, 71, 596-604, 1994.

 

           67.       Wu JR & Lentz BR: A Method for Quantitative Interpretation of Fluorescence Detection of Poly(ethylene glycol)-Mediated DPHpPC Transfer and Fusion Between Phospholipid Vesicles in the Dehydrated State. J. of Fluorescence 4, 153-163, 1994.

 

           68.       Lentz BR, Zhou C-M, Wu JR: Phosphatidylserine-Containing Membranes Alter the Thermal Stability of Prothrombin's Catalytic Domain: A Differential Scanning Calori­metric Study. Biochemistry 33, 5460-5468, 1994.

 

           69.       Lentz BR: Polymer-Induced Membrane Fusion: Potential Mechanism & Relation to Cell Fusion Events. Chem. Phys. Lipids, 73, 91-106, 1994.

 

           70.       Lentz, BR: Are Acidic Lipid Domains Induced by Extrinsic Proteins Binding to Membranes? Molecular Membrane Biol.,12, 65-67, 1995.

 

           71.       Lentz BR: Fluorescent Lifetimes of Diphenylhexatriene-Containing Probes Reflect Local Probe Concentrations: Application to the Measurement of Membrane Fusion. J. of Fluorescence 5, 29-38, 1995.

 

           72.       Koppaka V. & Lentz BR: Binding of Bovine Factor Va to Phosphatidylcholine Mem­branes. Biophys. J., 70, 2930-2937, 1996.

 

           73.       Cutsforth GA, Koppaka V, Krishnaswamy S, Wu JR, Mann KG, & Lentz BR: Insights into The Complex Association of Bovine Factor Va with Acidic-Lipid Contain­ing Synthetic Membranes. Biophys. J., 70, 2938-2949, 1996.

 

           74.       Chen Q, Lord S, & Lentz BR: Construction, Properties, and Specific Fluorescent Labeling of A Bovine Prothrombin Mutant Engineered with A Free C-Terminal Cysteine. Protein Engineering, 9, 545-553, 1996.

 

           75.       Koppaka, V, Wang, J-F, Banerjee, M, Lentz, BR: Soluble Phospholipids Enhance Factor Xa-Catalyzed Prothrombin Activation in Solution. Biochemistry, 35, 7482-7491 1996.

 

           76.       Wu, H, Zheng, L-X, & Lentz, BR: A Slight Asymmetry in the Transbilayer Distribution of Lysophosphatidylcholine Alters the Surface Properties and Poly(ethylene glycol)-Mediated Fusion of Dipalmitoylphosphatidylcholine Large Unilamellar Vesicles. Biochemistry, 35, 12602-12611, 1996.

 

           77.       Lentz, BR, Wu, JR, Zheng, L-X, & Prevrátil, J: The Interfacial Region of Dipalmitoylphosphatidylcholine Bilayers Is Perturbed by Fusogenic Amphi­paths. Biophys. J., 71, 3302-3310, 1996.

 

           78.       Lee J-K & Lentz BR: A Slight Outer Leaflet Perturbation Promotes PEG-Induced Fusion of Lipid Vesicles. Biochemistry, 36, 421-431, 1997.

 

           79.       Lentz, BR, Talbot, W, Lee, J-K, & Zheng, L-X: Transbilayer Lipid Redistribution Accompanies Poly(ethylene glycol) Treatment of Model Membranes but Is Not Induced by Fusion. Biochemistry, 36, 2076-2083, 1997.

 

           80.       Chen, Q, Lord, ST, & Lentz, BR: Partially Purified Echis Carinatus Venom Cleaves Active-Site-Mutated Bovine Prothrombin at Two Sites. Thrombosis Research, 85, 369-375, 1997.

 

           81.       Talbot, W, Zheng, L-X, & Lentz, BR: Acyl Chain Unsaturation and Vesicle Curvature Alter Outer Leaflet Packing and Promote Poly(ethylene glycol)-Mediated Membrane Fusion. Biochemistry,36, 5827-5836, 1997.

 

           82.       Chen, Q & Lentz, BR: A Fluorescence Resonance Energy Transfer Study of Shape Changes in Membrane-Bound Bovine Prothrombin and Meizothrombin. Biochemistry, 36, 4701-4711, 1997.

 

           83.       Lee, J-K & Lentz, BR: Evolution of Lipidic Structures During Model Membrane Fusion and The Relation of This Process to Cell Membrane Fusion. Biochemistry 36, 6251-6259, 1997.

 

           84.       Koppaka, V, Talbot, WF, Zhai, X., & Lentz, BR: Roles of Factor Va Heavy and Light Chains in Protein and Lipid Rearrangements Associated with the Formation of A Bovine Factor Va - Membrane Complex. Biophysical J. 72, 2638-2652, 1997.

 

           85.       Harper, MF, Hayes, PM, Lentz, BR, & Roubey, RAS: Characterization of b2-Glycoprotein I Binding to Phospholipid Membranes.  Thrombosis & Haemostasis, 80, 610-614, 1998.

 

86.              Lee, JK & Lentz, BR: Secretory and Viral Fusion May Share Mechanistic Events with Fusion between Curved Lipid Bilayers. Proc. Natl. Acad. Sci. USA, 95, 9274-9279, 1998.

 

           87.       Kim, SW, Ortel, TL, Quinn-Allen, MA, Yoo, L, Worfolk, L, Zhai, X, Lentz, BR, and Kane, WH: Partial glycosylation at asparagine-2181 of the second C-type domain of human factor V modulates assembly of the prothrombinase complex. Biochemistry, 38, 11448-11454, 1999.

 

88.       Lentz, BR & Lee, JK: Poly(ethylene glycol) (PEG)-Mediated Fusion between Pure Lipid Bilayers: A Mechanism in Common with Viral Fusion and Secretory Vesicle Release? A Review.  Membrane Molecular Biology, 16, 279-296, 1999.

 

89.       Lentz, BR: Commentary: Lipids and liposomes can do more than carry drugs; Phosphatidylserine as a regulator of blood coagulation.  Journal of Liposome Research, 9, IX-XV, 1999.

 

90.              Lentz, BR, Malinin, V, Haque, MdE, and Evans, K: Protein Machines and Lipid Assemblies: Currents Views of Cell Membrane Fusion.  Current Opinions in Structural Biology, 10, 607-615, 2000.

 

91.       Haque , MdE, McIntosh, TJ, Lentz, BR Influence of Lipid Composition on Physical Properties and PEG-mediated Fusion of Curved and Uncurved Model Membrane Vesicles: “Nature’s Own” Fusogenic Lipid Bilayer.  Biochemistry, 40, 4340-4348, 2001.

 

92.       Malinin, VS, Haque, MdE Lentz, BR: The Rate of Lipid Transfer During Fusion Depends on the Structure of Fluorescent Lipid Probes: A New Chain-Labeled Lipid Transfer Probe Pair.  Biochemistry, 40, 8292-8299, 2001.

 

93.       Srivastava, A, Quinn-Allen, MA, Kim, SW, Kane, WH, Lentz, BR: Soluble Phosphatidylserine (C6PS) Binds to a Single Identified Site in the C2-Domain of Human Factor Va.  Biochemistry, 40, 8246-8255, 2001.

 

94.       Presnell, SR, Tripathy, A, Lentz, BR, Jin, D-J, Stafford, DW: A Novel Fluorescence Assay To Study Propeptide Interaction with g-Glutamyl Carboxylase.  Biochemistry, 40, 11723-11733, 2001.

 

95.       Haque, ME, McCoy, AJ, Glenn, J, Lee, JK, Lentz, BR: Effects of Hemagglutinin “Fusion Peptide” on Poly(ethylene glycol)-Mediated Fusion of Phosphatidylcholine Vesicles.  Biochemistry, 40, 14243-14251, 2001.

 

96.       Wu, JR, Zhou, C-M, Majumder, R, Powers, DD, Weinreb, G, Lentz, BR: Role Of Procoagulant Lipids In Human Prothrombin Activation. 1: Prothrombin Activation By Factor Xa In The Absence Of Factor Va And In The Absence And Presence Membranes.  Biochemistry, 41, 935-949, 2002.

 

97.       Banerjee, M, Majumder, R, Weinreb, G, Wang, J-F, Lentz, BR: Role Of Procoagulant Lipids In Human Prothrombin Activation.  2: Soluble Phosphatidylserine Up-Regulates And Directs Factor Xa To Appropriate Peptide Bonds In Prothrombin.  Biochemistry, 41, 950-957, 2002.

 

98.       Srivastava, A, Wang, J-F, Rezaie, AR, Stenflo, J, Esmon, CT, Lentz, BR: Localization of Phosphatidylserine (C6PS) Binding Sites to Structural Domains of Factor Xa.  J. Biol. Chem., 277, 1855-1863, 2002.

 

99.       Malinin, VS, Frederik, P, Lentz, BR: Osmotic and Curvature Stress Affect PEG-Induced Fusion of Lipid Vesicles but Not Mixing of Their Lipids.  Biophys. J. 82, 2090-2100, 2002.

 

100.     Malinin, V.S. & Lentz, B.R.: Pyrene-Cholesterol Reports The Transient Appearance Of Non-Lamellar Intermediate Structures During Fusion Of Model Membranes.  Biochemistry, 41, 5913-5919, 2002.

 

101.    Zhai, X, Srivastava, A, Drummond, DC, Daleke, D, Lentz, BR: Phosphatidylserine binding alters the conformation and specifically enhances the cofactor activity of bovine factor Va. Biochemistry, 41, 5675-5684, 2002.

 

102.    Banerjee, M, Drummond, DC, Srivastava, A, Daleke D, and Lentz BR: Specificity of Soluble Phospholipid Binding Sites on Human Factor Xa.  Biochemistry, 41, 2751-62, 2002.

 

103.    Majumder, R, Weinreb, G, Zhai, Z, Lentz, BR: Soluble Phosphatidylserine Triggers Assembly in Solution of A Prothrombin-Activating Complex in The Absence of A Membrane Surface. Journal of Biological Chemistry, 277, 29765-29773, 2002.

 

104.    Haque, MdE and Lentz, BR: Influence of gp41 Fusion Peptide on the kinetics of Poly(ethylene glycol)-Mediated Model Membrane Fusion. Biochemistry, 41, 10866-10876, 2002.

 

105.    Evans, KO & BR: Kinetics of Lipid Rearrangements during Poly(ethylene glycol)-Mediated Fusion of Highly Curved Unilamellar Vesicles. Biochemistry, 41, 1241-1249, 2002.

 

106.    Majumder R, Wang, F-F, Lentz, BR: Effects of Water Soluble Phosphatidylserine on Bovine Factor Xa:  Functional and Structural Changes Plus Dimerization. Biophysical Journal, 84, 1238-1251, 2003.

 

107.    Dennison, SM, Greenfield, N, Lenard, J, Lentz, BR: VSV Transmembrane Domain (TMD) Peptide Promotes PEG-Mediated Fusion of Liposomes in a Conformationally Sensitive Fashion. Biochemistry, 41, 14925-14934, 2002.

 

108.    Weinreb, G, Mukhopadhyay, K, Majumder, R, Lentz, BR: Cooperative Roles of Factor Va and PS-Containing Membranes as Cofactors in Prothrombin Activation. Journal of Biological Chemistry, 278, 5679-5684, 2003.

 

109.    Malinin, VS and Lentz, BR: Energetics of Vesicle Fusion Intermediates: Comparison of Calculations with Observed Effects of Osmotic and Curvature Stresses. Biophysical Journal, 86, 3951-3964, 2004.

 

110.    Malinin, VS and Lentz, BR: On the Analysis of Elastic Deformations in Hexagonal Phases. Biophysical Journal, 86, 3324-3328, 2004.

 

111.    Haque, MdE and Lentz, BR: Roles of Curvature and Hydrophobic Interstice Energy in Fusion:  Studies of Lipid Perturbant Effects. Biochemistry, 43, 3507-3517, 2004.

 

Patents:

 

            1.         Lentz, BR, Majumder, R, Huang, JM: Soluble Phospholipids for Use in Clotting Factor Assays.  September 2003, US Preliminary Patent No. 5470-398PR

 

           In Textbooks and Review Volumes:

 

           1.         Thompson TE, Lentz BR, Barenholz Y:  A Calorimetric and Fluorescent Probe Study of Phase Transitions in Phosphatidylcholine Liposomes.  In "Biochemistry of Mem­brane Transport" [ed] G. Semenza and E. Carafoli, Springer-Verlag, Heidel­berg, 47-71, 1977.

 

           2.         Lentz BR:  Membrane "Fluidity" from Fluorescence Anisotropy Measurements.  In "Spectroscopic Membrane Probes" [ed] L.M. Loew, Vol. I, CRC Press, Inc, Boca Raton, FL, 1988, Chapter 2, pp. 13-41.

 

           3.         Lentz BR:  Organization of Membrane Lipids by Intrinsic Membrane Proteins.  In "Advances in Membrane Fluidity" [ed] R.C. Aloia, C.C. Curtain, L.M. Gordon, Alan R. Liss, Inc., New York, NY, 1988, 141-161.

 

           4.         Lentz BR, Burgess SW, Gratton E:  Concentration Dependence of DPHpPC Lifetime: Photophysics and Utility for Monitoring Membrane Fusion.  In "Molecu­lar Mecha­nisms of Membrane Fusion" [ed] S. Ohki, D. Doyle, T. Flanagan, S.W. Hui and E. Mayhew, Plenum Publishing Corp., New York, 1988, pp. 557-566.

 

           5.         Lentz, BR & Burgess, SW: Fluorescence Lifetime Measurements to Monitor Mem­brane Lipid Mixing. Meth. Enzymology, 1993, Vol. 220, Academic Press, Inc., NY, pp 42-50.

 

           6.         Lentz BR: Liposomes as a Tool to Study Blood Coagulation. in "Nonmedical Applica­tions of Liposomes, Volume II" [ed] Y. Barenholz & D.D. Lasic, CRC Press, Boca Raton, FL, 1995, pp 123-136.

 

           7.         Lentz BR, Siegel D, Malinin V: Filling Potholes on the Path to Fusion.  Biophysical Journal, 82, 2002, 555-557.

 

           8.         Lentz BR: Exposure of platelet membrane phosphatidylserine regulates blood coagulation.  Progress in Lipid Research, 42 2003, 423-438.

 

            Abstracts (most recent unpublished work only; these are all represented by manuscripts in some stage of preparation):

 

           81.       Banerjee, M, Koppaka, V., Zhou, C-M & Lentz, BR: Phosphatidylserine Binding Sites in Krigle Modules Regulate The Domain Organization and Conformation of Bovine Prothrombin. Biophyical J. 72: A307, 1997.

 

82.      Wang, J-F, Chen, Q & Lentz, BR: A Fluorescence Resonance Energy Transfer Study of Prothrombinase Assembly on Phosphatidylserine-Containing Membranes. Biophysical J 72: A307, 1997.

 

85.      Banerjee, M, Koppaka, V, Zhou, C-M, & Lentz, BR. Phosphatidylserine Binding Sites in Kringle Modules Regulate the Domain Organization and Conformation of Bovine Prthrombin. Thrombosis & Haemostasis, 1997.

 

86.      Wang, J-F, Chen, Q, & Lentz, BR. A Fluorescence Resonance Transfer Study of Prothrombinase Assembly on PS-Containing Membranes. Thrombosis & Haemostasis, 1997.

          

103.    Weinreb G. & Lentz, B.R. A Model to Predict the Dependence of Prothrombin Activation Kinetics on Lipid Concentration and Membrane Phosphatidylserine Content. Biophysical J. 78, A2466.

 

105.    Haque, E, Koppaka, V, Axelsen, PH, Lentz, BR: Relation between Bilayer Properties and Structures of HA and HIV Fusion Peptide-Membrane Complexes. Biophysical J. 82, 2002, A2640.

 

107.    Dennison, M, Bowen, M, Brunger, A, Lentz, BR: SNARE Proteins Alter Poly(ethylene glycol) (PEG)-Mediated Fusion of Model Membranes. Biophysical J. 84, 2003, A951.

 

108.    Majumder, R, Quinn-Allen, MA, Kane, WH, Lentz, BR: A Phosphatidylserine Binding Site Distinct from the C2 Domain Membrane Attachement Site Regulates the Assembly and Activity of the Human Prothrombinase. J. of Thrombosis & Haemostasis 1, 2004, Ap1048.

 

110.    Dennison, M & Lentz, BR: Effect of Free Ca2+ on the PEG-Mediated Fusion of Liposomes Having Synaptic-Vesicle-Like Composition. Biophysical J. 86, 2004, A230.

 

111.    Weinreb, G. & Lentz, BR: Kintetic Model of PEG-Mediate Vesicle Fusion.  Biophysical J. 86, 2004, A231.

 

           D.        CURRENT RESEARCH COLLABORATORS:

 

           Dr. Peter Frederik, Pathology Department, University of Masstricht, The Netherlands, Drs. Frederik and Lentz share an interest in the molecular mecha­nism of cell membrane fusion and are exploring ways to visualize this process using freeze fracture electron microscopy.

 

           Dr. David Daleke, Department of Chemistry, Indiana University, Bloomington, IN. Dr. Daleke is synthesizing short chain forms of phosphatidylserine analogues be used by Dr. Lentz's laboratory in screening for the specificities of regulatory sites on factor Xa and its cofactor, factor Va.  The hope is also to find compounds that will block prothrombin activation.

 

           Dr. Johan Stenflo, Clinical Chemistry Department, Univ. of Lund, Malmö General Hospital, Malmö Sweden.  Dr. Stenflo is supplying Dr. Lentz’s laboratory with selected fragments of factor Xa for efforts to locate the allosteric PS binding site in this serine protease.  A three way collaboration is also underway with Professors Torbjörn Drakenberg and Sture Forsén of the Physical Chemistry Center of Lund University to determine the structure of the PS-occupied site.

 

           Dr. Brian Edwards, Dept. of Biochemistry, Wayne State Univ., Detroit, MI.  Dr. Lentz’s laboratory is supplying Dr. Edwards with a peptide fragment containing the PS regulatory site from prothrombin.  Together, they are trying to obtain crystals of this peptide with and without co-crystallized PS, and are now trying to make heavy atom derivatives to assist in structure determination.

 

           Dr. William Kane, Hematology Division, Duke University School of Medicine, Durham, NC. Drs. Kane and Lentz are working together to locate and characterize the PS regulatory sites in blood coagulation factor Va, a critical component of the prothrombin activating complex.  A combination of mutational, enzymatic, and structural methods are being applied.

 

           Dr. Andreas Holzenburg, Lecturer in Structural Molecular Biology at the University of Leeds, School of Biochemistry & Molecular Biology, Leeds, UK.  Drs. Holzenburg, Stoylova (an Associate in Holzenburg’s laboratory), and Lentz are trying to locate conditions for obtaining 2D crystals of prothrombinase components on supported bilayers.  These will be used to obtained low resolution structures by X-ray and electron scattering methods.  Dr. Lentz is providing proteins and suggestions for crystalization conditions.

 

Drs. Ronald Bach (Minneapolis VA  Medical Center) and Yale Nemerson (Mt Sinai School of Medicine, NY, NY).  This collaboration will test the hypothesis that PS regulates the initiation of blood coagulation via the activation of factor Xa by regulating the dimerization state of tissue factor, an essential cofactor in this reaction.  Drs. Bach and Nemerson are supplying the tissue factor protein and their expertise in this reaction, while Dr. Lentz’s group is doing physical studies.

 

          

E.        SELECTED INVITED RESEARCH TALKS:

 

           "Structural Basis of PEG-Mediated Membrane Fusion", National Laboratory of Biomole­cules, Institute of Biophysics, Academia Sinica, Beijing, P.R. China, November 6, 1992.

 

           "Platelet Membranes & Control of Blood Coagulation", Dept. of Biophysics, Beijing Medical University, Beijing, P.R. China, November 7, 1992.

 

           "Platelet Membranes & Control of Blood Coagulation", International Workshop on Biomacro­molecules: Structure and Function, Wuxi, P.R. China, November 12-14, 1992.

          

           "Membrane Structure & Dynamics: A View of Polymer Induced Membrane Fusion", First Workshop on Physics of Complex Fluids & Biological Systems, Pohang Institute of Science and Technology (POSTECH), Pohang, Korea, March 24-26, 1993.

 

           "Platelet Membranes & Control of Blood Coagulation", POSTECH Life Science Depart­ment, Pohang, Korea, 3/23/93.

 

           "Platelet Membranes & Control of Blood Coagulation", Dept. of Chemistry, Seoul National Univ., Seoul, Korea, 3/27/93.

 

           "Measurement of Membrane Fusion Using Fluorescent Probes", Plenary lecture presented at The Third Conference on Methods and Application of Fluorescence Spectroscopy, Prague, Czech Republic, October, 1993.

 

           "Platelet Membranes and Control of Blood Coagulation", Biochemistry Dept., Cardiovas­cular Research Institute, Univ. of Limburg, Maastricht, The Netherlands, October, 1993.

 

           "The Role of Platelet Membranes in Prothrombin Activation to Thrombin Dur­ing Blood Coagulation", Univ. of Virginia, Department of Biochemistry, Charlottes­ville, VA, 2/17/94.

 

           "Are Acidic Lipid Domains Induced by Prothrombin Binding to Membranes?" Fogarty Conference on "Domain Organization in Membranes", NIH, Bethesda, MD, 3/2/94 - 3/4/94.

 

           "Platelet Phosphatidylserine Is An Allosteric Effector of Prothrombin Activation During Blood Coagulation", Ortho Diagnostic Systems, Inc., Raritan, NJ, 7/25/95.

 

           “Driving Force and Sequence of Events in PRG-Mediated Fusion of Phospholipid Vesicles”, "Mechanisms of Biological Membrane Fusion" satellite symposium held in conjunction with the International Congress of Pure and Applied Biophysics, August 8-10, 1996, Noorwijkerhout, The Netherlands.

 

           “Allosteric Regulation of Prothrombin Activation by Phosphatidylserine During Blood Coagulation.”  Univ. of Missouri School of Biological Science, Kansas City, MO, 10/31/96.

 

           “PEG-Mediated Membrane Fusion: Driving Force, Sequence of Events, and Relationship to Viral or Exocytotic Fusion.”  Univ. of Virginia Molecular Biophysics Seminar, Charlottesville, VA, 11/11/96.

 

           “Allosteric Regulation of Prothrombin Activation by Platelet Membrane Phosphatidylserine.”  1] Temple Univ. Biochemistry Dept., 12/13/96.

2] Univ. of Pennsylvania Vascular Biology Series, 12/12/96.

3] Thomas Jefferson Univ. Cardeza Foundation for Hematologic Research, 12/11/96, Philadelphia, PA.

 

           “PEG-Mediated Model Membrane Fusion: Structural Events and Their Relation to Events During Biomembrane Fusion.”  Dept. Cell Biology & Anatomy, UNC School of Medicine, Chapel Hill, NC, 2/12/97.

 

“PEG-Mediated Model Membrane Fusion: Structural Events and Their Relation to Events During Biomembrane Fusion.”  Laboratory for Theoretical Biology, National Cancer Institute, NIH, Bethesda, MD, 2/19/97.

 

“The Roles of Phospholipid in the Functioning of Vitamin K-Dependent Proteins.”  FASEB Summer Conference on Vitamin K, Saxtons River, VT, 8/2/97-8/7/97.

 

           “Platelet Membrane Phosphatidylserine as a Second Messenger and Allosteric Regulator of Blood Coagulation.”  Dept. of  Biochemistry, Molecular & Cell Biology, Univ. of Kansas, Lawrence, KA, Sept. 26, 1997.

 

            “Effects of Fusion Peptide on PEG-Mediated Vesicle Fusion.” Symposium honoring Prof. T.E. Thompson, Univ. of Virginia, Charlottesville, Virginia, Nov. 6-7, 1997.

 

           “Platelet Membrane Phosphatidylserine as a Second Messenger and Allosteric Regulator of Blood Coagulation.”  Clinical Chemistry Dept. and Physical Chemistry Division of the Chemical Center, Univ. of Lund and Malmö, Sweeden, Nov. 17 & 18, 1997.

 

           “Platelet Membrane Phosphatidylserine as a Second Messenger and Allosteric Regulator of Blood Coagulation.” Biochemistry Department, Wayne State University, Detroit, MI, May 19, 1998.

 

           “Fusion between Pure Lipid Bilayers: A Mechanism in Common with Viral and Exocytotic Fusion.”  “Membrane Fusion: Mechanisms and Applications to Cell Biology, Drug Delivery, and Gene Therapy.” Symposium, Salamanca, Spain, July 14-18, 1998.

 

“Peg-Mediated Fusion Between Pure Lipid Bilayers: Proposed Mechanism, Role Of PEG, Relation To Biomembrane Fusion.” The Society for Physical Regulation in Biology and Medicine, “Cell Membrane Sealing and Fusion” Symposium.  Long Beach CA, November 12, 1998.

           

“Platelet Phosphatidylserine (PS) Is an Allosteric Regulator of Factors Xa and Va And a Key Second Messenger During Blood Coagulation.” February 13, 1999. Membrane Structure and Assembly Subgroup Symposium, Biophysical Society Meeting, Baltimore, MD.

 

“PEG-mediated lipid bilayer fusion: Steps in common with viral fusion and secretory vesicle release.”  April 23, 1999. Molecular Biophysics & Biochemistry Dept., Yale Univ., New Haven, CT.

 

“PEG-mediated lipid bilayer fusion: A Mechanism in common with secretory and viral fusion.”  Materials Research Society Annual Meeting, Nov. 30, 1999, Boston, MA.

 

“Research At The Interface Between The Biomedical Sciences And Physics, Chemistry, Mathematics.” Presented to RISE/MBRS Program students and faculty, Cayey University College, Cayey, Puerto Rico, March 30, 2000.

 

“A View of Cell Membrane Fusion: Protein Machines Work on Lipid Materials.” FASEB Summer Conference on Molecular Biophysics of Cellular Membranes.  Saxtons River, VT, July 15-20, 2000.

 

“Poly(ethylene glycol)-Mediated Vesicle Fusion: A Mechanism in Common with Exocytotic and Viral Fusion.” Presented at a Juan March Institute international workshop on “Comparison of the mechanisms of cellular vesicle and viral membrane fusion”, Madrid, November 26- 29, 2000. 

 

“Mechanism of Membrane Fusion: Protein Machines Work on Lipid Structures.” Presented at Sixth Annual Life and Physical Science Research Symposium, North Carolina A&T University, Greensboro, NC, 2/23/01.

 

“Protein machines and Lipid Assemblies: Current Views of Cell Membrane Fusion.” Department of Biochemistry, North Carolina State University, Raleigh, NC, 3/22/01.

 

“Regulation of Vitamin K Protein Function by Phospholipid.” FASEB Conference, Saxtons River, VT, August 4-9, 2001.

 

“Filling the Potholes on the Road to Fusion.” Dept. of Physiology and Biophysics, Robert Wood Johnson Medical School, Piscatawy, NJ, February 18, 2002.

 

“Effects of Fusion Proteins on the Kinetics of Model Membrane Fusion.” Symposium talk, 2002 Biophysical Society Meeting in San Antonio, TX.

 

“Mechanism of Membrane Fusion: Protein Machines Work on Lipid Structures.” Morgan State University Biomedical Research Seminar Series, March 13, 2003, Baltimore MD.

 

“Efficient Human Thrombin Generation Requires Molecular Phosphatidylserine and Not a Membrane Surface.” July 17, 3003, ICTH Meeting in Manchester UK.

 

“How Might Proteins Drive Fusion? Filling Potholes on the Path to Fusion.” Chemistry Department, Univ. of Maryland, Baltimore Co., November 18, 2003, Baltimore MD.

 

“Phosphatidylserine: A Second Messenger in Regulating Blood Coagulation” Chemistry Department, University of North Carolina at Chapel Hill, November 5, 2003.