Rinku Majumder
E.mail :rinku_majumder@med.unc.edu
r_majumder@hotmail.com
Business Address
Home Address
University of North Carolina at Chapel Hill
600 Airport Road,
Department of Biochemistry, CB# 7260
Northampton Plaza, Apt#515,
Chapel Hill, NC, 27599, USA
Chapel Hill, NC, 27514, USA
Tel: 919-962-8317, Fax: 919-966-2852
Tel: 919-968-8183
CAREER OBJECTIVE
R&D in pharmaceutical, Biotechnology, diagnostic or cosmetics industry.
SKILL SUMMARY
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Biochemist with a strong working background in protein
biochemistry, molecular biology, analytical spectroscopy, biophysical chemistry
and enzyme kinetics.
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BIOCHEMICAL: Protein isolation and purification; Enzyme
assay; Ion exchange chromatography; gel filtration; SDS and native polyaccrylamide
gel electrophoresis; paper chromatography and TLC; HPLC to separate proteins,
small molecules, polysachharide moiety.
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SPECTROSCOPIC: Ultraviolet and visible absorption;
Circular dichroism; Light scattering; Fluorescence microscopy; Steady state
fluorescence; Time resolved fluorescence; Nano second fluoremeter; Stop
flow kinetics; Rapid Quench flow kinetics;
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MOLECULAR BIOLOGY: cDNA library construction and screening;
vector construction and cloning of gene; Plant nuclear DNA and RNA isolation;
Plasmid DNA isolation; Transformation of bacteria; Western blot; ELISA
and other commonly used microbial techniques.
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COMPUTATIONAL: Experience with MS-DOS and MS-Windows
operating systems; Conversant with most of the software useful for word
processing and data processing in DOS and windows environments; (Word perfect;
SigmaPlot, etc.)
EDUCATION
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Ph.D. in Biochemistry (1998), Bose Institute, Calcutta, India.
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DISSERTATION: Physicochemical Characterization of a
unique Apyrase from M.pudica and cloning of its gene. Thesis involved isolation,
characterization of the enzyme Apyrase & constructing the cDNA library
of Mimosa pudica, cloning and sequencing of Apyrase gene. While purifying
and characterizing this enzyme, it was observed that a chromophore is associated
with it, having absorption in the ultraviolet-A/blue region of the spectrum.
The chromophore has been identified as N5, N10-Methenyl tetrahydrofolate
(MTHF) by comparing with synthetic MTHF (prepared by me by chemical synthesis)
and also reconstituting the enzyme with synthetic MTHF. Upon excitation
with light (350 nm), an increase of apyrase activity was observed in the
purified or reconstituted holoenzyme but not in the apoenzyme.
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M.S. in Biochemistry with First Division (1991), Kalyani
University, West Bengal, India.
EMPLOYMENT HISTORY
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Research Associate, The University of North Carolina
at Chapel Hill, February 2001-present
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Investigating protein-protein and protein-membrane interactions involving
in the prothrombinase reaction in the blood coagulation system
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Post-doctoral Research Associate, The University of North Carolina
at Chapel Hill, May 98- January 2001
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Investigating protein-protein and protein-membrane interactions involving
in the prothrombinase reaction in the blood coagulation system
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Pre-doctoral Fellow, Bose Institute, Calcutta, India, September 92-April
98. Isolated, characterized the protein Apyrase from the motile plant
Mimosa pudica and cloned and sequenced the gene of this novel and unique
protein.
HONORS/AWARDS AND SCHOLARSHIPS
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Qualified in the nationwide test, GRADUATE APTITUDE TEST FOR ENGINEERING,
(GATE), February 1992
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Invited speaker at All India Cell Biology Conference at Calcutta,
India, Feb 1996.
PROFESSIONAL MEMBERSHIPS
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Member: American Biophysical Society,
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Life member: Indian Chemical Society.
PUBLICATIONS
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Rinku Ghosh, S. Biswas and S. Roy: An apyrase from Mimosa pudica
contains N5, N10-methenyl tetrahydrofolate and is stimulated by light.
Eur.J.Biochem. 1998, 258: 1009.
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Rinku Ghosh, P.Sen and S.Biswas: Mimosa pudica apyrase requires polysaccharide
and Ca2+ for the activity. Molecular and Cellular Biochemistry. 1998, 187:
47.
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Rinku Majumder (Ghosh), Gabriel Weinreb, Xin Zhai & Barry R.Lentz.
Soluble Phosphatidylserine Triggers Assembly in Solution of a Prothrombin-
Activating Complex in the Absence of a Membrane Surface. J.Biol.Chem.
2002, 277:29765-73
Rinku Majumder* (Ghosh), Jiangfang Wang* and Barry R.Lentz: Functional
and structural consequences of water-soluble phosphatidylserine binding
to bovine factor Xa.( Submitted the revised version to Biophysical journal).
*The first two authors contributed equally to this work.
Rinku Majumder (Ghosh), G. Weinreb and Barry. R. Lentz: Role of Phosphatidylserine
in enhancing the rate of human prothrombin activation by the prothrombinase
complex in solution. Manuscript in preparation.
Jogin R Wu, Chaoming Zhou, Rinku Majumder (Ghosh), Daniel D Powers,
Gabriel Weinreb and Barry R. Lentz : Role of procoagulant membranes in
human prothrombin activation. 1: Prothrombin Activation by factor Xa in
the absence of factor Va and in the absence and presence of membranes.
Biochemistry 2002 Jan 22; 41(3): 935-49
Mou Banerjee*, Rinku Majumder (Ghosh)* , Gabriel Weinreb* and Barry
R. Lentz: Role of procoagulant lipids in human prothrombin activation.
2: Soluble phosphatidyl serine up regulates and directs factor Xa.to appropriate
peptide bonds in prothrombin. Biochemistry 2002 Jan 22; 41(3): 950-957
? The first three authors contributed equally to this work.
Arvind Srivastava, Jianfang Wang, Rinku Majumder (Ghosh), Alireza R.
Rezaie, Johan Stenflo, Charles T. Esmon and Barry R. Lentz: Localization
of phosphatidyl serine (C6PS) binding sites to structural domains of factor
Xa.J Biol Chem. 2002 Jan 18; 277(3): 1855-63.
G. Weinreb*, K. Mukhopadhyay*, R. Majumder (Ghosh)*, and B.R. Lentz.
The Cooperative Roles of Membranes and Factor Va in the Membrane-Bound
Prothrombinase Complex. 2002 (submitted the revised copy to Biochemistry).
*The first three authors contributed equally to this work.
CONFERENCE PRESENTATIONS
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Rinku Majumder, X.Zhai and B. R. Lentz Soluble Phosphatidylserine
(C6PS) binding to a FVa alters its ability to bind FXa and to function
in the formation of thrombin during blood coagulation, New Orleans Feb.
2000.
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Rinku Ghosh and Susweta Biswas Mimosa pudica apyrase needs calcium and
polysaccharide for the activity,. International Union of Biochemistry and
Molecular Biology (IUBMB) at New Delhi, India, Sept. 1994.
REFERENCES
Barry R. Lentz Ph.D. Professor and Director of Molecular
and Cellular Biophysics, CB #7260, 418 MEJB, University of
North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. Tel: (919) 966
5384, Fax: (919) 966 2852,
E-mail: uncbrl@med.unc.edu
A.Lahiri Majumder, Ph.D. Professor of Department
of Plant molecular & Cellular Biology, Bose Institute, Calcutta India.
Tel: (33) 3379416, E-mail: lahiri@bic.boseinst.ernet.in
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